About Us

We are a premier Contract Research and Development Organization (CRDO) at the forefront of biologics manufacturing innovation. Our High-throughput Antibody Production Service is a specialized platform designed to meet the demanding needs of modern drug discovery, diagnostic development, and functional proteomics. Leveraging state-of-the-art automation, advanced parallel bioreactor systems, and streamlined downstream processing workflows, we produce hundreds to thousands of distinct antibody variants in parallel—delivering milligram quantities of each within a condensed timeframe. This service is the critical bridge between initial antibody discovery (e.g., from phage display or single B cell sequencing) and the rigorous in vitro and early in vivo validation that requires tangible, purified protein. We partner with pharmaceutical companies, antibody platform developers, and academic consortia to accelerate their pipelines by providing rapid, reliable, and scalable access to the essential reagents needed to make informed go/no-go decisions.

☞Service Highlights

Our High-throughput Antibody Production Service is engineered to deliver unparalleled speed, consistency, and data-rich outputs.

• Unmatched Parallel Processing Capacity: Our automated platform can concurrently handle 96 to 384+ unique antibody expression projects in a single campaign, from small-scale transfections to parallel micro-purifications.

• Rapid Turnaround Time: We deliver purified, QC’d antibody samples in 4-6 weeks, dramatically accelerating the transition from sequence to functional data compared to traditional one-at-a-time production methods.

• Integrated, Automated Workflow: From automated plasmid preparation and cell seeding to robotic liquid handling for transfection, feeding, and harvest, our process minimizes manual error and maximizes reproducibility across all samples in a batch.

• Milligram-Scale Yields: We produce 1-10 mg of purified antibody per variant—a quantity sufficient for extensive characterization, including affinity measurement (SPR/BLI), epitope binning, specificity profiling, and preliminary functional or cell-based assays.

• Standardized & Data-Driven: Every sample undergoes a standardized QC pipeline. You receive a uniform dataset for all antibodies, enabling direct, apples-to-apples comparison to rank leads objectively.

• Seamless Integration with Discovery: This service is the ideal next step after high-throughput antibody discovery platforms. We efficiently accept and process large sequence panels, codon-optimize them in batch, and manage the entire production pipeline.

☞High-throughput Antibody Production Technology Process

Our technology integrates parallelized upstream and downstream processes into a seamless, automated pipeline.

 Phase 1: High-Throughput Vector Preparation & Cell Culture 

• Batch Cloning & Sequence Management: Your panel of antibody sequences (e.g., from NGS output of a selection campaign) is received. Sequences are batch-codon-optimized and synthesized or cloned in parallel into standardized expression vectors (e.g., mammalian IgG vectors).

• Automated Transfection in Multi-Format: We utilize high-density cell culture platforms (e.g., 24-deep well plates, 96-well blocks, or miniature bioreactor arrays). Using automated liquid handlers, HEK293 or CHO suspension cells are transfected in parallel with the array of expression constructs.

• Parallel Fed-Batch Cultivation: Cultures are maintained in automated incubator-shakers. Robotic systems administer standardized feed solutions to each culture individually or in groups, optimizing cell growth and protein titers in a miniaturized, parallel format over 7-14 days.

 Phase 2: Micro-Scale Harvest & Parallel Purification 

• Automated Clarification: Post-expression, cultures are processed in parallel using centrifugation or micro-filtration plates to remove cells, yielding clarified supernatants containing the secreted antibodies.

• Micro-Purification Chromatography: This is the core of our throughput. We employ:

1. Robotic Protein A/G Affinity Capture: Using 96-well filter plates pre-packed with affinity resin or automated liquid handlers coupled to micro-columns, we perform parallel capture and elution of IgGs from all supernatants simultaneously.

2. High-Throughput Buffer Exchange: Using centrifugal filter plates or automated dialysis, purified samples are simultaneously buffer-exchanged into a standard storage formulation (e.g., PBS).

 Phase 3: Parallelized Analytics & Quality Control 

• Automated Quantification: The concentration of every purified sample is determined in parallel using a microplate-based spectrophotometer (A280) or a fluorescence-based protein assay (e.g., NanoOrange).

• Rapid Purity Assessment: Samples are analyzed by parallel capillary electrophoresis (e.g., Caliper LabChip® or CE-SDS) or microfluidic SDS-PAGE systems, providing digital data on purity and molecular weight for dozens of samples in under an hour.

• High-Throughput Binding Confirmation: A mandatory QC step involves testing each antibody's binding activity via a high-throughput ELISA or Octet/BLI assay in a 96- or 384-well format to confirm functionality before release.

 Phase 4: Data Compilation & Sample Management 

• All analytical data is automatically captured, linked to the sample barcode, and compiled into a master database.

• Purified antibodies are dispensed into barcoded microtubes or plates in pre-defined aliquots, ready for shipment or direct integration into your downstream screening assays.

Figure1.Quality control of recombinant antibody repertoires by high‐throughput sequencing.

☞Our Service Details & Workflow

Our client-facing process is designed for ease of use, scalability, and clear communication when managing large-scale projects.

Step 1: Project Design & Consultation

We discuss the scale of your project (number of sequences), antibody format (IgG, Fab, etc.), required yield per clone, and any special requirements. A detailed project plan, timeline, and quote are provided.

Step 2: Sequence Submission & Vector Logistics

You provide the panel of antibody variable region sequences in a specified format. We manage the batch gene synthesis (if needed), codon optimization, and high-throughput cloning into our production vectors. Alternatively, you may provide pre-cloned plasmids.

Step 3: Parallel Expression & Production Campaign

Our automated platform initiates the parallel expression run. You receive a campaign initiation report and a mid-point update on transfection and early titer metrics.

Step 4: Purification, QC & Data Delivery

The batch undergoes parallel purification and analytics. Upon completion, you receive:

• Physical Deliverables: All purified antibody samples (typically 0.5-2 mL at 0.5-2 mg/mL), aliquoted and formatted as requested (individual tubes or plate-based).

• Digital Deliverables: A comprehensive data package for every sample, including concentration, capillary electrophoresis purity profile (gel-like image and quantification), and binding activity data (e.g., ELISA OD values). Data is provided in both PDF reports and machine-readable formats (e.g., .csv).

Step 5: Shipment & Project Close-out

The final product package is shipped on dry ice. A project close-out meeting can be arranged to review the data and discuss potential next steps, such as down-selection to a smaller panel for larger-scale production.

 Our High-Throughput Service Workflow: 

☞Classification of High-throughput Antibody Services

Our service can be tailored to different stages of the pipeline and antibody types:

①By Production Scale & Purpose:

• Micro-Production (μg to 1 mg): For initial screening, epitope binning, and cross-reactivity profiling immediately following discovery.

• Milligram-Scale Production (1-10 mg): Our standard service, ideal for detailed characterization (kinetics, specificity panels), assay development, and early functional testing.

②By Antibody Format:

• High-Throughput IgG Production: Full-length monoclonal antibodies in mammalian systems (HEK293 transient, CHO pools).

• High-Throughput Antibody Fragment Production: Parallel production of scFv, Fab, or VHH fragments in E. coli or yeast systems, offering even faster turnaround and lower cost per variant.

• High-Throughput Bispecific Format Production: Production of common bispecific formats (e.g., IgG-scFv, knob-into-hole) from paired heavy and light chain sequences.

③By Integration Level:

• Standalone Production Service: We produce your provided panel of sequences.

• Integrated Discovery-to-Production Service: We couple our service with upstream phage display or single B cell screening, offering a seamless pipeline from library screening to purified lead panels.

☞Applications of High-throughput Antibody Production

The ability to rapidly generate numerous purified antibodies transforms multiple areas of biologics research and development:

• Antibody Discovery & Lead Selection: Following a phage display or immunization campaign, produce hundreds of unique hits in parallel to quickly identify the top 5-10 leads based on real protein performance (expression, stability, binding), not just sequence data.

• Epitope Binning & Mapping: Generate a panel of antibodies against a target to perform high-throughput competition assays (e.g., on the Octet platform) to group antibodies by epitope, informing intellectual property strategy and therapeutic combination potential.

• Diagnostic Assay Development: Rapidly produce and screen hundreds of candidate capture and detection antibody pairs to identify the optimal matched pair for a sensitive and specific sandwich ELISA or lateral flow assay.

• Antibody Engineering & Optimization: Produce and test libraries of engineered variants (e.g., for affinity maturation, humanization, or stability improvement) to gather structure-activity relationship (SAR) data efficiently.

• Functional Screening: Provide the tangible reagents needed for medium-throughput cell-based assays, such as antibody-dependent cellular cytotoxicity (ADCC), receptor blocking, or neutralization assays, to prioritize leads with the desired biological function.

• Protein Microarray Generation: Produce a diverse array of antibodies for immobilization on chips to create custom protein microarrays for proteomic screening or biomarker validation.

Accelerate your antibody pipeline from sequences to actionable data. Contact our team to discuss your high-throughput production needs and leverage our automated platform to make faster, more informed decisions in your biologics development.