Nebulabio is a protein engineering and production team specializing in recombinant protein expression in mammalian systems for research and preclinical discovery workflows. By integrating construct strategy, host selection, and fit-for-purpose process development, we deliver proteins with clear documentation, traceable QC, and reproducible performance across production runs. The working principle is straightforward: clients provide the gene-of-interest (GOI) and intended protein format, and we deliver a mammalian expression solution—together with a complete data package—aligned to the downstream assay needs.
☞Nebulabio’s Bacterial Expression Platform
Bacterial systems, most commonly based on E. coli, are widely used for recombinant protein production due to rapid growth, mature genetic toolkits, and strong yield potential. Our platform provides an end-to-end route spanning gene design readiness, vector construction, expression screening, scale-up production, purification, and characterization. Projects are executed under defined milestones so that expression decisions are evidence-based and protein deliverables remain consistent across runs.
| ☎ +86-15801534258 | ✉ info@nebulabio.cn | Contact Us ☜☜ |
☞Supported Expression Options
| Host Strains | Expression Vectors and Tags |
• BL21(DE3) • Origami or trxB gor mutants • Rosetta or Tuner strains • Custom strains for project-defined needs | • Inducible promoters including T7 lac trc araBAD • Tags including His GST MBP SUMO FLAG Strep II • Periplasmic targeting signals including PelB OmpA DsbA • Cleavage sites including TEV Thrombin Factor Xa |
| Expression and Production Modes | Purification and Refolding Coverage |
• Small scale condition screening • Shaker flask or bioreactor scale-up • Auto induction option when appropriate | • Affinity capture and polishing steps • Inclusion body solubilization and refolding when required • Optional endotoxin reduction for specific downstream contexts |
| Quality Control | |
• SDS PAGE Western blot • SEC HPLC when configured • Identity support by mass spectrometry when configured • Activity or binding assays when project-defined |
☞Principle of Bacterial Protein Expression
Bacterial expression produces recombinant proteins by introducing a plasmid carrying the gene of interest under a regulated promoter into a prokaryotic host such as E. coli. Upon induction, the bacterial transcription and translation machinery synthesizes the target protein, which may remain in the cytoplasm, be directed to the periplasm through signal peptides, or accumulate as inclusion bodies depending on sequence properties and expression conditions. While bacterial systems do not perform most eukaryotic post-translational modifications, they are widely used for enzymes, antigens, and non-glycosylated proteins where speed, yield, and cost-efficiency are primary constraints.
☞Nebulabio Service Workflow
Project Design and Construct Engineering We translate your gene and protein format requirements into a buildable bacterial expression strategy suitable for downstream purification and characterization. Key Steps: • Goal definition including protein size solubility expectations tag requirements yield tier and timeline • Codon optimization and gene readiness review for E. coli contexts • Vector selection and cloning plan aligned with promoter tag and antibiotic marker • Sequence verification to confirm insert integrity |
Small Scale Expression Screening We evaluate expression and solubility across a controlled set of conditions to establish a data-supported route for scale-up. Key Steps: • Transformation into selected expression strains • Condition screening across induction temperature and time windows • Solubility assessment and basic identity support by SDS PAGE and Western blot when configured |
Scale Up Production We produce the target protein at the requested scale under the selected condition set and document key process checkpoints. Key Steps: • Seed train preparation from defined starter material • Shaker flask or bioreactor cultivation with induction timing control • Harvest and lysis followed by clarification of soluble fraction or inclusion body collection |
Purification and Refolding We isolate the target protein to the agreed purity tier and implement refolding coverage when inclusion body recovery is required. Key Steps: • Affinity capture aligned with tag choice • Optional tag removal with corresponding polishing • Ion exchange or size exclusion polishing when configured • Inclusion body solubilization and refolding route when required |
Quality Control and Characterization We generate a traceable QC dataset that supports identity purity and project-defined functional attributes. Key Assays: • Purity support by SDS PAGE and optional SEC HPLC • Concentration determination using standard quantification methods • Identity support by Western blot or mass spectrometry when configured • Activity or binding assessment when project-defined • Endotoxin quantification when requested |
Delivery and Documentation We deliver aliquoted protein material and a complete documentation package so results can be reproduced and audited. Deliverables: • Purified protein aliquots in defined storage buffer • COA and QC summary with associated raw data • Handling and storage guidance including reconstitution notes when relevant |
☞Featured Applications of Nebulabio’s Services
● Research Reagents and Enzymes
Bacterial expression is widely used for routine supply of enzymes and research proteins where glycosylation is not required. It supports rapid iteration for variant sets and enables standardized production for assay development and benchmarking.
● Diagnostic Antigens and Calibrators
Non-glycosylated antigens and epitope-containing fragments are commonly produced in E. coli for immunoassay contexts such as ELISA development. Standardized batch characterization supports comparability across studies and reagent lots.
● Structural Biology and Crystallography
E. coli expression supports production of proteins for crystallography and isotope labeling contexts where defined sequence variants and homogeneous preparations are required. It is also compatible with standard identity and purity characterization frameworks prior to structural work.
● Vaccine Candidate Antigens
Subunit antigens and immunogenic fragments can be produced rapidly in bacterial systems for early material availability and comparative testing of antigen formats. Characterization focuses on identity and consistency of the produced constructs.
For more information, please contact us at info@nebulabio.cn or +86-15801534258.
