About Us
Nebulabio is an antibody discovery and engineering team specialized in isolating and cloning antigen-specific monoclonal antibodies directly from single B cells. By integrating high-parameter flow cytometry, microfluidic single-cell handling, and single-cell V(D)J sequencing with recombinant expression and analytical validation, we deliver sequence-defined antibodies with native heavy–light chain pairing and traceable documentation. This service supports antibody discovery programs that require repertoire-faithful binders, clear clonal lineage records, and standardized downstream verification.
Nebulabio Single B Cell Sorting Platform
The platform provides direct access to native B cell repertoires by isolating individual antigen-binding cells from immunized, vaccinated, convalescent, or engineered donors. Fluorescent antigen probes and multi-parameter phenotyping enable selection of defined B cell subsets, while single-cell sequencing recovers paired variable regions that are then reconstituted as recombinant antibodies in configured formats. The workflow can accommodate memory B cell–focused strategies, plasmablast enrichment, and hybridoma rescue when project-defined, with downstream expression in mammalian systems for consistent molecular assembly.
☞Supported Options
Sample Types
| Antigen Probes and Labeling
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Sorting and Isolation Technologies
| Downstream Processing
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Screening and Characterization
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| ☎ +86-15801534258 | ✉ info@nebulabio.cn | Request a Quote ☜ |
☞Principle of Single B Cell Sorting
Single B cell sorting is a repertoire-faithful antibody discovery route that preserves native heavy–light chain pairing at the level of individual antigen-binding cells. Antigen-specific cells are identified using labeled probes combined with phenotypic markers and isolated as single cells under controlled conditions. Immunoglobulin variable regions are recovered from each cell and reconstructed as recombinant antibodies, yielding monoclonals that reflect in vivo affinity maturation and immune selection. This approach enables rapid recovery of sequence-defined binders to conformational antigens and complex targets while maintaining a clear clone-to-phenotype record.
☞Nebulabio Service Workflow
Project Design and Antigen Probe Preparation We establish the selection logic, configure probe chemistry, and define the downstream confirmation plan. Key Steps
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B Cell Staining and Single-cell Isolation We stain cells with antigen probes and phenotype markers and isolate single antigen-binding B cells by configured technology. Key Steps
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Single-cell V(D)J Recovery and Sequence Annotation We recover paired variable regions and generate a sequence-defined clone set. Key Steps
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Construct Cloning and Recombinant Expression We clone paired sequences into configured formats and express antibodies for primary confirmation. Key Steps
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Binding Confirmation and Characterization We verify binding and specificity and generate project-defined characterization datasets. Key Steps
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Delivery and Documentation We deliver antibodies, sequences, and traceable documentation for downstream work. Deliverables
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☞Featured Applications of Single B Cell Sorting
◆ Therapeutic Antibody Lead Discovery
Single-cell recovery from immunized or convalescent donors enables rapid identification of naturally matured binders with native pairing. This route supports generation of diversified candidate panels that can be reformatted for downstream developability evaluation.
◆ Diagnostic Antibody Development
Native antibodies against disease biomarkers can be discovered directly from clinical samples, enabling development of highly specific immunoassay reagents with minimal off-target binding.
◆ Antibody Engineering and Humanization
Fully human sequences obtained from transgenic animals or human donors reduce immunogenicity risks and simplify downstream engineering for improved developability.
◆ Infectious Disease and Vaccine Research
Antibodies from convalescent or vaccinated donors provide insights into protective epitopes and support the design of vaccine immunogens and therapeutic cocktails.
◆ Autoimmune and Tolerance Studies
Isolation of self-reactive B cells helps characterize autoimmune antibody repertoires and identify targets for therapeutic intervention or tolerance induction.
| For more information, please contact us at info@nebulabio.cn or +86-15801534258. |
