About Us

Nebulabio is a specialized provider of high-quality, custom polyclonal antibody (pAb) discovery and production services. With extensive experience in immunology and antibody development, we partner with researchers and organizations worldwide to generate robust, target-specific polyclonal antisera for diverse applications in research, diagnostics, and early-stage development. We recognize that polyclonal antibodies remain indispensable tools due to their high affinity, ability to recognize multiple epitopes, and suitability for detecting denatured proteins. Our mission is to deliver reliable, consistent, and high-titer antisera by combining scientific expertise with stringent project management and animal welfare standards. We offer fully customizable protocols—from antigen design and animal immunization to serum purification and characterization—ensuring you receive reagents that significantly enhance the sensitivity and reliability of your assays.

☞Technologies for Polyclonal Antibody Discovery

Nebulabio’s platform leverages a combination of proven methodologies and optimized protocols to maximize immune response and antibody quality.

①Comprehensive Animal Host Options: We offer immunization in a variety of host species to match your project needs, including rabbit, mouse, rat, goat, sheep, and chicken (for IgY production). Rabbits are our most common host due to their strong immune response and relatively large serum yield.

②Expert Antigen Design & Preparation: Success begins with a well-designed immunogen. Our services include:

• Antigen Consultation: Advising on optimal immunogen selection (full-length protein, peptides, fragments).

• Carrier Protein Conjugation: We expertly conjugate small peptides and haptens to immunogenic carriers (KLH, BSA, OVA) using various chemistries (e.g., via cysteine lysine residues).

• Adjuvant Selection: We employ standard (Freund’s) and specialty adjuvants to enhance immune response while minimizing animal discomfort, tailored to the antigen and host species.

③Optimized Immunization Protocols: Our protocols are designed to elicit a strong, specific, and sustained antibody response. This includes:

• Customized immunization schedules (dose, route, number of boosts).

• Pre-immune serum collection as a critical negative control.

• Regular test bleeds to monitor antibody titer and specificity via ELISA.

④Purification & Processing Options: We provide a range of purification services to meet specific application requirements:

• Total IgG Purification: Using Protein A/G or antigen-specific affinity purification.

• Peptide-AntiSerum (PAS) Purification: Affinity purification against the specific peptide immunogen to increase specificity.

• IgY Purification from Egg Yolk: A non-invasive alternative for avian hosts.

• Serum processing, sterile filtration, and preservation.

Figure1. Approaches used to produce current clinically approved Polyclonal antibodies.

☞Nebulabio’s Service Process & Workflow

Our process is transparent, collaborative, and designed for reliability from start to finish.

 Step-by-Step Workflow: 

• Project Consultation & Design: We discuss your target, desired applications, and required specifications (host, purity, volume). Together, we finalize the antigen strategy and project plan.

• Antigen Preparation: Our lab prepares or quality-controls the immunogen, performing conjugation if necessary. We provide a certificate of analysis.

• Animal Immunization & Monitoring: Animals are housed in an AAALAC-accredited facility under strict ethical guidelines. The customized immunization schedule begins, with test bleeds analyzed to monitor progress.

• Final Production Bleed: Once the target titer is achieved, a final large-volume bleed is performed to collect the maximum volume of antiserum.

• Serum Processing & Purification: The final bleed is processed (clotted, centrifuged). Purification is performed according to the selected service package.

• Quality Control & Characterization: Every batch undergoes essential QC, including titer verification by ELISA, specificity assessment (e.g., Western blot against target), and concentration measurement.

• Delivery & Documentation: The final product is shipped along with a comprehensive report detailing the immunization protocol, purification method, and all QC data.

☞Applications of Polyclonal Antibodies

Polyclonal antibodies produced through our services are powerful tools in numerous fields:

• Research & Biomarker Detection: Ideal for Western Blotting and Immunohistochemistry (IHC), where their ability to recognize multiple epitopes enhances detection sensitivity, especially for low-abundance or denatured targets.

• Diagnostic Development: Widely used as capture and detection antibodies in enzyme-linked immunosorbent assays (ELISAs), lateral flow assays, and other diagnostic platforms due to their strong signal amplification.

• Immunoprecipitation (IP) & Chromatin IP (ChIP): Their high affinity and multi-epitope binding make them exceptionally effective for pulling down target proteins and associated complexes from biological lysates.

• Neutralization & Functional Studies: Polyclonal antisera can contain antibodies that block protein function or receptor-ligand interactions, useful for in vitro neutralization assays.

• Secondary Antibody Production: Our anti-species polyclonal antibodies (e.g., anti-rabbit IgG produced in goat) are essential reagents used as secondary detectors in countless immunoassays.

☞Why Choose Nebulabio?

• Proven Expertise & High Success Rate: Our extensive experience in antigen design and immunization strategy minimizes project risk, ensuring a high rate of success in generating specific, high-titer antisera.

• Full Customization & Expert Guidance: We offer true flexibility at every step—no rigid packages. Our scientists provide consultative support to design the optimal project for your goals and budget.

• Ethical Standards & Animal Welfare: We are committed to the highest standards of animal welfare. All work is conducted in accredited facilities adhering to strict ethical guidelines, ensuring responsible and humane treatment.

• Superior Quality & Comprehensive Data: We don’t just deliver a vial of serum. Each project includes detailed QC data (titer, specificity blot), giving you confidence in the reagent’s performance and ensuring reproducibility in your experiments.

• Transparent Process & Project Management: You receive regular updates on immunization progress and test bleed results. A dedicated project manager ensures clear communication and timely delivery.

• One-Stop Solution: From antigen design to purified antibody, we manage the entire process, saving you time and laboratory resources while guaranteeing consistency.

Partner with Nebulabio for your polyclonal antibody needs and leverage our commitment to quality, ethics, and scientific partnership to obtain reliable, high-performance reagents that accelerate your research and development timelines.