Nebulabio is a premier global provider of integrated Custom Protein Services, offering a comprehensive suite of solutions that span from gene design and recombinant expression to sophisticated purification and chemical synthesis. With over 15 years of specialized experience, we serve as an essential partner to pharmaceutical companies, biotechnology innovators, and academic research institutions worldwide. Our state-of-the-art facilities and multidisciplinary team of molecular biologists, protein chemists, and synthetic chemists enable us to tackle the most challenging protein projects—whether it's expressing a difficult membrane protein, purifying a multi-subunit complex to homogeneity, or synthesizing a long, modified peptide. We are committed to delivering not just a product, but a fully characterized, high-quality protein or peptide that meets your exact specifications, accelerating your research, diagnostic development, or therapeutic program.
☞Protein Expression System Classification
The foundation of successful recombinant protein production is selecting the optimal expression host. We offer expertise across all major systems, each with unique advantages tailored to different protein types and end-use requirements.
Expression System | Typical Hosts | Key Advantages | Best Applications |
Prokaryotic | E. coli (e.g., BL21, Rosetta strains) | Fastest & most cost-effective; high yields for soluble proteins; extensive genetic tools; easy scale-up. | Cytosolic proteins, enzymes, antibody fragments (scFv, Fab), antigens for immunization, proteins for structural studies. |
Yeast | Pichia pastoris, Saccharomyces cerevisiae | High-cell density fermentation; secretes protein; performs basic eukaryotic PTMs (e.g., disulfide bonds, glycosylation). | Secreted proteins, disulfide-rich proteins, some enzymes, and proteins requiring eukaryotic folding but not complex glycosylation. |
Insect Cell / Baculovirus | Sf9, Sf21, High Five™ cells | Ideal for large, complex, multi-subunit proteins; performs most eukaryotic PTMs; high expression levels. | Protein complexes (e.g., kinases, channels), viral antigens, membrane proteins (GPCRs), proteins requiring eukaryotic-specific folding. |
Mammalian Cell | HEK293, CHO, ExpiCHO™ | Gold standard for human therapeutics; authentic human post-translational modifications (PTMs) including complex glycosylation; ensures proper folding and bioactivity. | Therapeutic antibodies, complex glycoproteins, receptors, proteins where authentic human PTMs are critical for function. |
Cell-Free | E. coli or Wheat Germ extracts | Fastest from gene to protein (hours); ideal for toxic proteins, incorporation of unnatural amino acids, and isotope labeling. | High-throughput screening, toxic proteins, protein labeling for NMR, small-scale functional testing. |
| ☎ +86-15801534258 | ✉ info@nebulabio.cn | Request a Quote ☜ |
☞Technical Introduction to Protein Production, Purification, and Synthesis
Nebulabio’s integrated service platform covers the complete journey from sequence to pure, functional protein or peptide.
A. Recombinant Protein Production & Purification
This is our core service for producing proteins encoded by a DNA sequence.
Construct Design & Vector Engineering: Your gene is codon-optimized for the chosen host and cloned into an expression vector. We strategically incorporate tags (His, GST, Fc, SUMO) to aid solubility, detection, and purification.
Expression Screening & Optimization: We perform small-scale expression trials to identify the best construct and conditions (temperature, inducer, media) for maximizing soluble yield.
Scale-Up Production: The optimized protocol is scaled in shake flasks or bioreactors under controlled conditions (pH, O₂, feeding).
Purification Pipeline:
Capture: Affinity chromatography (e.g., IMAC for His-tag, Protein A for Fc-fusions) isolates the target.
Tag Cleavage: Removal of fusion tags using specific proteases (e.g., TEV, HRV 3C).
Polishing: Ion exchange (IEX), hydrophobic interaction (HIC), and size exclusion (SEC) chromatography remove impurities, aggregates, and fragments.
Formulation: The pure protein is concentrated and exchanged into your specified buffer.
B.Peptide & Small Protein Chemical Synthesis
For short sequences (typically up to ~70 amino acids) or those containing non-natural modifications, we offer chemical synthesis.
Solid-Phase Peptide Synthesis (SPPS): We primarily use Fmoc (9-fluorenylmethoxycarbonyl) chemistry, building the peptide chain stepwise on a solid resin. This allows for the incorporation of unnatural amino acids, isotopic labels (e.g., ¹⁵N, ¹³C), site-specific modifications (phosphorylation, acetylation, PEGylation), and fluorescent dyes.
Cleavage & Deprotection: The full-length peptide is cleaved from the resin and side-chain protecting groups are removed.
Purification & QC: Crude peptides are purified to >95% purity using reverse-phase high-performance liquid chromatography (RP-HPLC). Identity is confirmed by mass spectrometry (MALDI-TOF or LC-MS), and purity is assessed by analytical HPLC.
☞Nebulabio Service Workflow
Our client-centric workflow is designed for seamless collaboration, transparency, and successful project execution from initial concept to final delivery.
Step 1: Project Consultation & Quotation
Contact us with your protein/peptide sequence, desired quantity, purity, modifications, and intended use. We provide a detailed project proposal outlining the recommended strategy, timeline, and cost.
Step 2: Project Initiation & Sequence Confirmation
Upon agreement, the project is initiated. For recombinant projects, we proceed with gene synthesis and cloning. For synthetic peptides, the sequence and modifications are finalized.
Step 3: Production & Purification/Synthesis Execution
Recombinant Path: Expression screening, scale-up, and the multi-step purification process are executed.
Synthesis Path: Solid-phase synthesis, cleavage, and purification are performed.
Step 4: Quality Control & Analytical Characterization
The final product undergoes rigorous QC:
Purity: SDS-PAGE, SEC-HPLC (proteins) or Analytical RP-HPLC (peptides).
Identity: Mass Spectrometry.
Concentration: UV spectrophotometry or amino acid analysis.
Functionality (Optional): Binding assays (ELISA, SPR), enzymatic activity, or other client-specified tests.
Step 5: Final Delivery & Reporting
You receive the purified protein or peptide, a comprehensive Certificate of Analysis (CoA), and a detailed project report. All data, including chromatograms and spectra, are included.
Our Integrated Service Workflow:
☞Applications of Custom Proteins
The high-quality proteins and peptides produced through our services are fundamental tools across diverse fields:
Drug Discovery & Preclinical Research:
Target Proteins: Production of purified enzymes, receptors, and ion channels for high-throughput screening (HTS) and structure-based drug design.
Therapeutic Antibodies & Proteins: Development and production of candidate biologics for in vitro and in vivo studies.
Structural Biology & Biophysics: Providing homogeneous, monodisperse proteins for X-ray crystallography, cryo-EM, and NMR studies.
Diagnostic Development:
Antigens & Calibrators: Producing recombinant antigens or synthetic peptides for immunoassays (ELISA, lateral flow) and as reference standards.
Antibody Production: Generating immunogens for raising antibodies in animals.
Vaccine Research: Production of recombinant subunit vaccine antigens and virus-like particles (VLPs).
Cell & Gene Therapy: Manufacturing critical protein factors (growth factors, cytokines) used in cell culture and differentiation protocols.
Basic Research: Supplying specific proteins or modified peptides to study signaling pathways, protein-protein interactions, and post-translational modification effects.
Partner with Nebulabio for a single-source solution to your most demanding protein needs. From gene to vial, or from sequence to purified peptide, our Custom Protein Service delivers the quality and expertise required to advance your science.
For more information, please contact us at info@nebulabio.cn or +86-15801534258.
