About Us
Nebulabio is a specialized antibody discovery and engineering team focused on generating chicken-derived monoclonal antibodies for research, assay development, and translational antibody programs. Avian immunization offers a practical route to high-affinity binders against highly conserved mammalian proteins, peptides, and conformational epitopes that often show weak immunogenicity in rodents. By integrating avian B cell isolation, single-cell V(D)J sequencing, and recombinant expression, we deliver fully chicken monoclonal antibodies with native pairing, defined specificity, and documentation that supports traceable downstream use.
☞Nebulabio Chicken Antibody Platform
Chickens generate antibody diversity through gene conversion and somatic hypermutation in the bursa of Fabricius, creating a repertoire that is often complementary to mammalian repertoires. We access antigen-specific B cells from immunized birds, naturally exposed or convalescent flocks, and project-defined naïve sources. Chicken-optimized antigen probes and multi-parameter sorting enable isolation of single antigen-binding cells, followed by single-cell sequencing to recover paired variable regions. Recombinant production is configured in mammalian, avian, insect, or microbial hosts depending on the antibody format, with support for full-length IgY and recombinant fragments such as Fab and scFv.
☞Supported Options
Sample Types • PBMCs from immunized or naïve chickens • Splenocytes from immunized birds • Bone marrow aspirates (project-dependent) • Bursa of Fabricius-derived B cells (project-dependent) • Cryopreserved or fresh whole blood • Egg yolk–associated sources upon request (project-dependent) | Immunization and Donor Strategies • Active immunization of purpose-bred chickens with defined antigens • Convalescent donors after natural exposure or challenge models • Naïve repertoire mining from healthy flocks • In vitro priming approaches for low-immunogenicity or toxic targets (project-dependent) • Display library construction from chicken repertoires (project-dependent) | |
Antigen Probes and Labeling • Recombinant proteins configured for probe preparation • Cell-surface staining using antigen-expressing mammalian or avian cells • Inactivated viral particles or pathogen preparations (project-dependent) • Biotinylated antigens with fluorescent streptavidin conjugates • Peptide–MHC multimers for TCR-like binders (project-dependent) | Isotype and Format Support • Full-length chicken IgY and configured IgY variants • Chicken IgA and IgM when configured • Recombinant scFv and Fab formats • Single-domain derivatives when configured • Isotype-specific secondary reagents for detection and capture |
☞Principle of Chicken-derived Antibody Production
Chicken antibody diversification differs from mammals, relying heavily on gene conversion in the bursa of Fabricius and subsequent somatic hypermutation during affinity maturation. This evolutionary divergence enables robust responses against epitopes that are conserved in mammals and therefore less immunogenic in rodent hosts. The platform captures antigen-specific chicken B cells through probe-based single-cell isolation or configured library routes, recovers paired variable region sequences via chicken-optimized amplification and sequencing, and reconstitutes antibodies in recombinant formats suited to downstream assays. The resulting antibodies retain the binding specificity of the original avian immune response while enabling consistent production and format flexibility.
| ☎ +86-15801534258 | ✉ info@nebulabio.cn | Request a Quote ☜ |
☞Nebulabio’s Service Process
Project Design and Antigen Probe Preparation We define the discovery route, configure antigen probe formats, and align evaluation assays with the intended downstream context. Key Steps
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Immunization and B Cell Enrichment We generate or source chicken immune material and enrich viable B cell populations suitable for single-cell workflows. Key Steps
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Antigen-specific Single-cell Isolation or Library Route We isolate antigen-binding chicken B cells by high-parameter sorting, or build display libraries from enriched repertoires when configured. Key Steps
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Single-cell Sequencing and Paired-chain Discovery We recover paired variable regions using chicken-optimized primer sets and bioinformatic annotation. Key Steps
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Construct Engineering, Expression, and Purification We clone paired sequences into IgY or configured recombinant formats and produce antibodies in selected hosts with format-appropriate purification. Key Steps
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Delivery and Documentation We deliver purified antibodies and documentation supporting reproducible downstream use. Deliverables
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☞Featured Applications
■ Highly Conserved Mammalian Antigens
Chicken-derived antibodies are frequently selected against conserved mammalian proteins where rodent immunization yields limited diversity. The phylogenetic distance can broaden epitope coverage and generate orthogonal binder panels useful for comparative reagent validation.
■ Immunoassay Reagent Development
IgY-based reagents can reduce assay interference associated with mammalian Fc contexts in certain sample matrices, supporting cleaner background profiles in configured immunoassay designs. Recombinant formats allow consistent production and standardized QC for method development workflows.
■ Food Safety and Pathogen Detection
Chicken antibodies are suitable as capture or detection binders in assays targeting foodborne pathogens and contaminants, where stability and scalable supply are practical considerations. Sequence-defined clones support reproducible performance benchmarking across assay iterations.
■ Avian Infectious Disease Research
Species-matched chicken monoclonals support antigen mapping, serological assay calibration, and mechanistic studies in poultry-relevant contexts. This is especially useful when experimental interpretation benefits from avian isotype background and defined clone identity.
| For more information, please contact us at info@nebulabio.cn or +86-15801534258. |
