About Us

Nebulabio is a pioneer in high-throughput (HT) recombinant antibody production, offering an industrialized platform to accelerate the generation of hundreds to thousands of purified, sequence-defined antibodies in parallel. We specialize in transforming genomic or synthetic antibody libraries into tangible, high-quality protein reagents at unprecedented speed and scale. Our service is designed for the next generation of large-scale biological research and discovery, serving needs in proteomics, functional genomics, therapeutic antibody screening, and diagnostic panel development. By integrating automated molecular cloning, parallelized mammalian expression (HEK293 and CHO systems), and robotic liquid handling with miniaturized purification workflows, we eliminate the traditional bottlenecks of antibody generation. Our mission is to empower large-scale functional studies, multiplexed assay development, and rapid hit-to-lead progression by providing a reliable, scalable pipeline for antibody production that matches the pace of modern omics and drug discovery campaigns.

☞High-throughput Antibody Production Process

Our core process is a seamlessly integrated, parallelized workflow engineered for maximum throughput without sacrificing quality. It leverages automation at every stage to ensure consistency and speed.

 ①HT Gene Synthesis & Cloning: 

• Input: Hundreds to thousands of antibody variable region (VH/VL) sequences, provided by the client or derived from sequencing campaigns (e.g., from single B cell or phage display projects).

• Process: Sequences are codon-optimized for mammalian expression. Using automated liquid handlers, genes are synthesized or PCR-amplified in 96- or 384-well plate formats and simultaneously cloned into our standard mammalian expression vectors (e.g., containing a CMV promoter and a constant region for IgG, Fab, or scFv format).

• Output: A verified, high-density plate library of antibody expression constructs.

 ②HT Transfection & Expression: 

• Platform: We utilize transient transfection in HEK293 cells as our primary HT workhorse for its rapid turnaround (7-10 days) and proven ability to produce correctly folded, glycosylated antibodies. For projects requiring higher titers or specific glycoforms, we offer parallelized stable pool generation in CHO cells.

• Process: Expression constructs are transfected in parallel into cells cultured in deep-well blocks or mini-bioreactors. Our automated systems handle complex transfection mixes, media changes, and feeding schedules across hundreds of cultures simultaneously.

• Output: Culture supernatants containing secreted antibodies, arrayed in microplates.

 ③HT Capture & Purification: 

• Technology: We employ high-throughput affinity purification methods. This often involves automated plate-based Protein A/G capture using filter plates or magnetic beads, or parallelized micro-column chromatography systems.

• Process: Supernatants are processed in parallel to bind, wash, and elute antibodies directly into a collection plate. Buffer exchange into a standard storage formulation (e.g., PBS) is performed concurrently using desalting plates or dialysis membranes.

• Output: A plate containing purified antibodies in a ready-to-use buffer.

 ④HT Quality Control & Analytics: 

• Analysis: A representative sample from each purification is analyzed in a high-throughput manner. Standard QC includes:

• Concentration: Measured via UV absorbance in plate readers.

• Purity & Integrity: Assessed by automated capillary electrophoresis (CE-SDS) or microfluidic gel systems.

• Functionality: Initial binding can be screened via automated ELISA or flow cytometry against the target antigen.

• Output: A comprehensive data matrix linked to each antibody well, detailing yield, purity, and initial binding data.

Figure1.Quality control of recombinant antibody repertoires by high‐throughput sequencing. 

☞Service Highlights

• Unmatched Speed & Scale: Produce 100s to 1000s of antibodies in 4-8 weeks, a timeline impossible with traditional sequential methods. This massively parallel approach accelerates project timelines by orders of magnitude.

• Automation-Driven Consistency & Reproducibility: By minimizing manual intervention, we ensure unprecedented lot-to-lot and clone-to-clone consistency in yield, purity, and performance. Automated pipetting eliminates human error and variability.

• True "Sequence-to-Protein" Pipeline: We accept raw sequence lists as input and deliver purified, QC-checked proteins as output, managing the entire complex process internally. This provides a single point of contact and accountability.

• Cost Efficiency at Scale: The economies of scale achieved through parallel processing and miniaturization significantly reduce the per-antibody production cost compared to traditional single-project fees, making large-scale studies economically viable.

• Flexible Formats & Scales: Services are offered in flexible batch sizes (96, 384, 1536-well formats). We provide material from microgram (for initial screening) to milligram (for assay development) scales per antibody.

• Integrated Data Management: Each antibody is tracked with a unique identifier. All associated data—sequence, vector map, expression titer, QC profile—are delivered in a structured, searchable format, enabling seamless integration into your data analysis pipelines.

☞Nebulabio’s Service Process

Our client engagement process is streamlined for clarity and efficiency, from project design to data delivery.

• Step 1: Project Onboarding & Strategy. We consult on project goals, finalize the antibody sequence list, and agree on format (IgG, Fab), expression system, scale, and QC requirements.

• Step 2: High-Throughput Cloning. Your sequences are processed through our automated gene synthesis and cloning pipeline to generate the expression-ready DNA library.

• Step 3: Parallelized Expression. The DNA library is used in our scaled, parallel transfection and expression platform.

• Step 4: Automated Purification. Antibodies are purified in parallel using our robotic HT purification systems.

• Step 5: High-Throughput QC. Each antibody undergoes rapid, automated quality control. Data is compiled in real-time.

• Step 6: Final Data & Material Delivery. You receive the physical plates of purified antibodies, along with a complete digital data package including sequences, yields, purity profiles, and any screening data.

☞Applications of High-throughput Antibody Production

This service is transformative for fields that require large, defined antibody sets:

• Therapeutic Antibody Screening: Rapidly express and functionally screen hundreds of lead candidates from discovery campaigns (e.g., from phage display, single B cell) to identify top performers for detailed characterization, drastically speeding up the hit-to-lead stage.

• Proteome-Scale Research & Protein Arrays: Generate antibodies against thousands of human proteins for use in functional protein microarrays, systematic localization studies (immunofluorescence), or interaction mapping, enabling systems-level biology.

• Diagnostic & Assay Development: Produce large panels of antibodies for multiplexed diagnostic assay development (e.g., cytokine panels, biomarker verification for liquid biopsy, multi-pathogen detection) to identify optimal matched pairs.

• Affinity Reagent Generation for CRISPR/Cas Screening: Produce antibodies for intracellular targets to validate hits from genetic screens via flow cytometry or immunofluorescence, adding a proteomic layer to genomic data.

• Antigen-Specific B Cell Receptor (BCR) Repertoire Analysis: Clone and express the paired antibodies from BCR sequencing studies to functionally characterize the immune response at scale, linking sequence to actual antigen binding.

• Cell Line Development Support: Generate dozens of candidate recombinant antibodies in parallel for early-stage testing before committing resources to a single clone for large-scale CHO cell line development.

☞Power Your Large-Scale Discovery

Unlock the potential of your antibody sequence data with Nebulabio’s industrial-scale, high-throughput production platform. Move beyond one-at-a-time cloning and expression to a new paradigm of speed, scale, and data-rich output.

Contact us today to discuss your large-scale project, request a quote, or schedule a technical consultation to explore how our platform can accelerate your research pipeline.